In a game played on a 4G surface at Doncaster Rovers' Keepmoat Stadium due to the poor weather, the Iron Under-18 side were edged out 3-2 by their Lincoln City counterparts on Saturday.
United had a couple of the second year trainees back so were a little bit stronger, but still had numerous Under-16 and Under-15 players involved.
Playing against the wind in the opening half, Scunthorpe started slowly and Lincoln got their first goal in the fifth minute when a long diagonal ball was played and an Imps player got in behind to open the scoring.
The Iron tried to get back into the game, and Cameron Wilson, who returned to the team, cut in from the right and had a shot which was deflected for a corner on 10 minutes.
United's passing wasn't the best and they kept giving the ball away, but their first real good chance came when they did manage to get the ball down and combine some passes together. Harrison Poulter crossed the ball for Trialist A, who headed the ball just over.
Poulter had a chance on 30 minutes when Wilson crossed the ball and his effort went narrowly wide.
The same players combined for another chance, as Wilson ran with the ball and passed to Poulter, but an away defender managed to get the ball away just as the Iron forward looked to pull the trigger inside the box.
Lincoln made it 2-0 on 40 minutes as they seized on a pass across the box, and nipped in to score.
With 71 minutes played, the Imps scored a penalty to make it 3-0.
From that point, Scunthorpe started to play and Trialist B had a chance, after being played through. He chipped the goalkeeper but a defender recovered and cleared the ball off the line.
Wilson scored in the 75th minute, winning a penalty before getting up and taking it to make it 3-1.
United pushed, and Ben Franklin shot just over afterwards.
The Iron managed to get another goal on 88 minutes to make it 3-2, with Jamie Moore netting. United won back possession and he managed to shoot through bodies in the area.
Scunthorpe had a couple of late efforts, including a dangerous free-kick on the edge of the box but Wilson's shot was saved by the goalkeeper.
Professional Development Phase Coach Steve Thornber said: "In the second-half we were much better, getting back into the game and showed more desire. In the first we were poor though."
IRON: Balme, Franklin, Gallimore, Kemp, Lewis (Strouther, 35), Lobley, Wilson, Moore, Cribb (Trialist B, 60), Poulter, Trialist A (Abraham, 72).
IRON UNUSED SUB: Crosher.