The Iron are odds-on 8/13 favourites to register three points in Group G as they take on Middlesbrough's development side.
Coach Paul Jenkins brings his Boro academy to Glanford Park as underdogs, according to the league's official betting partners Sky Bet, with them priced at 4/1 while a draw is 29/10.
Of course, in the latter scenario, both teams will be awarded a point, but a penalty shoot out will be undertaken to determine which side is awarded a bonus point.
United are 15/8 to win to nil, while Middlesbrough are 10/1. Elsewhere, it's 5/1 for Scunthorpe to net four or more goals and 16/1 to net five or more goals. Middlesbrough are priced at 40/1 and 150/1 respectively to achieve the same feat.
For more great odds, specials and accumulators visit skybet.com/scunthorpe.
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