The Iron survived numerous scares before emerging as victors in the Round of 128 match against Charlton Athletic last time out.
United streamed the game on their brand new Twitch channel, with a total of 4,700 views during the streaming session. Concurrent views peaked at over 600 as George Miller and Kevin van Veen netted in the 2-1 win against the Championship outfit to set up a Round of 64 tie.
Fellow League Two side Walsall came out with arguably the shock of the round as they overhauled AS Roma, who were labelled as the competition favourites by the EFL's betting partner Sky Bet. Meanwhile, Melbourne Victory possibly provided the comeback of the round as they came from 3-1 down to win 4-3 in stoppage time against Ipswich Town.
Elsewhere, organisers Leyton Orient were dumped out in the very first tie to be played, losing 6-3 to Lokomotiv Moscow. Angers embarrassed Waterford 10-0 in the greatest victory of the round, as did FC Emmen who beat Port Vale. Also amongst the biggest scoreline winners was Mansfield Town who beat Gent 6-1, while Feyenoord overhauled Orlando City 5-0.
United could draw the following teams in the Round of 64:
- Lokomotiv Moscow
- West Ham United
- FC Midjtylland
- Manchester City
- Southend Unitd
- FC Sion
- Cheltenham Town
- Standard Liege
- Angers
- Sheffield Wednesday
- Queens Park Rangers
- RB Salzburg
- Fortuna Sittard
- Fiorentina
- Newcastle United
- Preston North End
- Sheffield United
- Feyenoord
- Emmen
- Mansfield Town
- Stevenage
- Vitesse
- PSV
- Hamilton Accies
- Exeter City
- Reading
- Nantes
- Forest Green Rovers
- Brentford
- Sunderland
- Istanbul B
- Rotherham United
- Middlesbrough
- Dundalk
- VVV Venlo
- Lorient
- Sligo Rovers
- Milton Keynes Dons
- Norwich City
- Wycombe Wanderers
- Celta Vigo
- Central Coast Mariners
- ESTAC Troyes
- Millwall
- Ross County
- FC Sion
- West Bromwich Albion or Ajax
- Sydney
- Grimsby Town or Motherwell
- Adelaide United or Wolverhampton Wanderers
- LOSC Lille
- Crawley Town or RKC Waalwijk
- Stoke City or Blackburn Rovers
- Finns Harps
- Groningen
- ADO Den Haag
- Genk
- Rochdale or Coventry City
- Sparta Rotterdam
- Amiens
- Istanbul Basaksehir
- Utrecht
- Burnley