The Iron will play Gainsborough on Tuesday, July 18th (7.30pm kick-off) as part of the side's preparations for the 2023-24 season.
United will take on former midfielder Neal Bishop's side at the Northolme as both sides prepare for the 2023-24 campaign.
Trinity are looking for another successful season under Bishop, with the team narrowly missing out on promotion to Vanarama National League North after being edged out in their play-off semi-final encounter against Bamber Bridge on penalties.
Their gaffer, who has clocked around 800 appearances in his playing career, still assumes the role of Player-Manager, and will give Jimmy Dean's side a stern test with just two-and-a-half weeks of pre-season remaining at that point.
Harry Lewis, now of Cleethorpes, and current players Fin Shrimpton and Ty Sellars-Fleming enjoyed valuable match action at the Northolme last season, with the latter two in line to come up against the side they played for on loan during spells last season.
For the Iron, it will be the first meeting between the two sides since the Lincolnshire Senior Cup Final in April 2022 with goals from Harvey Cribb and Alfie Beestin seeing Scunthorpe lift the trophy in a 2-1 win.
Tickets for the encounter will be £10 for adults, £5 for concessions and £2 for under-18s and can be purchased here.
PRE-SEASON SCHEDULE CONFIRMED SO FAR...
Monday, June 26th - Players and staff return for pre-season training and testing
Tuesday, July 11th - Winterton Rangers (A) - 7.30pm
Tuesday, July 18th - Gainsborough Trinity (A) - 7.30pm