Jimmy Dean will be looking for a third win on the bounce following convincing victories over Ilkeston Town and Gainsborough Trinity, but face stern opposition from the EFL.
Doncaster Rovers, managed by former players of the Iron - Grant McCann and Cliff Byrne - will bring their side to see how far United have come in pre-season following a draw at Brigg Town CIC and a defeat at Winterton Rangers.
With Ian Lawlor, Tommy Rowe, George Miller and Caolan Lavery in their squad, Scunthorpe are likely to come up against some familiar faces at the weekend.
For the Iron, Reagan Ogle returned to action in midweek to play his first 45 minutes of pre-season. Tom Pugh returned to training on Thursday having sat out of recent games, while Dion Sembie-Ferris has stepped up his return following a minor operation to remove a growth on his foot.
The game comes two weeks ahead of the start of the Vanarama National League North campaign.
The referee for the encounter is Matthew Dicicco, while the assistants are Liam Smith and Dave Holmes.