United entered their home Checkatrade Trophy group stage game against Lincoln City making seven changes from the side that faced Luton Town in League One at the weekend.
Jak Alnwick, Jordan Clarke, Cameron Burgess, Cameron Borthwick-Jackson, Funso Ojo, Josh Morris and Lee Novak were replaced by Jonathan Flatt, Levi Sutton, Charlie Goode, Lewis Butroid, James Perch, Clayton Lewis and Andy Dales.
Scunthorpe United: Flatt, Lund, Humphrys, Dales, Perch, Lewis, Goode, Sutton, McArdle, Butroid, Colclough.
Substitutes: Alnwick, Clarke, Borthwick-Jackson, Ojo, Novak, Burgess, Pugh.
Lincoln City: Slocombe, Gordon, Wharton, Pett, Rhead, Green, Chapman, Wilson, Mensah, Crookes, Anderson.
Substitutes: Smith, Frecklington, Andrade, Toffolo, Akinde, Adebayo-Smith, Sartorius.