The Iron make nine changes entering their Checkatrade Trophy group stage home clash against Sunderland Under-21s.
Rory Watson starts in goal, while Jordan Clarke, Charlie Goode, Cameron Burgess (skipper for the night) and Lewis Butroid form a completely altered defence.
Hakeeb Adelakun (who started at the weekend), Sam Mantom, Devonte Redmond and Duane Holmes form the midfield, with Kevin van Veen partnering Tom Hopper (who also started at Plymouth) in attack.
Scunthorpe United: Watson, Clarke, Mantom, van Veen, Hopper, Adelakun, Redmond, Holmes, Goode, Burgess, Butroid.
Substitutes: Gilks, Townsend, Wallace, Ojo, Madden, Morris, Sutton.
Sunderland Under-21s: Talbot, Love, Matthews, Beadling, Hume, Molyneux, Bale, Rodwell, Robson, Greenwood, Gooch.
Substitutes: Storey, Wood, Wright, Taylor, Gamble, Allan, Diamond.