The Iron's U18 side exited the FA Youth Cup after a 4-2 home loss to Tranmere Rovers.
The visiting side had the first chance of the game on two minutes, as Tom Croughan fired over from just inside the penalty area.United's first chance arrived in the fifth minute, as George Barmby brilliantly turned his man, before dragging his shot just wide of the goal.
With seven minutes on the clock, Tranmere full back James Galloway's cross to the back post needed tipping over the crossbar by Iron 'keeper Adam Kelsey, who did well.
Just a minute later, Scunthorpe took the lead. Barmby opened the scoring with a neat finish from close range.
On 10 minutes, there was excellent defending from Lewis Butroid, as he shielded the ball out of play for a goal kick as Tranmere threatened.
In the 11th minute, Tranmere levelled, with Callum Lucy drilling the ball into the bottom corner of the goal.
Just three minutes later, McMichael did superb to prevent a second for the visitors, as he cleared a goal-bound shot off the line.
On 16 minutes, Scunthorpe went 2-1 up, as Hornshaw did well to square the ball for Barmby to tap home into an empty net.
Just two minutes after, there was a big chance for the visitors, but Croughan could only strike over from inside the box, after a cross from the left.
Kelsey did well to stop Tranmere levelling for a second time, as he pushed away a shot from Lucy.
There was a Scunthorpe chance on 27 minutes as Barmby went in search of his hat-trick, bringing down a cross-field ball, ahead of seeing his shot blocked.
Just moments later, Sithole fired a left footed strike from just inside the area. The effort took a deflection, but drifted just wide.
Chances fizzled out as the opening period wore on, with United defending well and stifling the visiting attackers.
In the 50th minute, Dean-Atkinson did superbly down the right flank, before standing the ball up for Barmby, whose header was blocked.
With 54 minutes on the clock, Tranmere levelled for a second time, as Udoyen Akpan slotted home neatly into the bottom corner of the goal.
Croughan then attempted to complete the turnaround with a long range drive. Kelsey had everything behind it though, and gathered.
On 58 minutes, there was great play from Barmby, who nutmegged his man, before sliding a perfectly weighted ball into the path of Hornshaw, whose shot was bravely charged down however.
In the 63rd minute, the visiting team took the lead when Declan Drysdale headed home from a corner.
Five minutes later they extended their lead, as Callum Lucy's left footed strike found the top corner of the net, as the visitors extended their lead.
In the 70th minute, Barmby crashed an effort off the foot of the post, as he was cruelly denied his hat-trick following decent build-up play.
Two minutes later, Akpan had an opportunity at the other end though but struck his effort wide of the post.
There were three Scunthorpe substitutions in the closing stages but the game gradually petered out as Tranmere won the right to visit Everton in the third round.
Scunthorpe United: Kelsey, McMichael, Butroid, Porter, Lilley, Sackey, Busby, Hornshaw, Barmby (Bartholomew, 81), Dean-Atkinson (Ablett, 76), Sithole (Train, 72).
Substitutes: Parker, Colclough.
Tranmere Rovers: Wharton, Galloway (Solomon-Davies, 48), Clarke, Lucy, J. Devine, DrysdalThe, Heron, E. Devine, Akpan, Croughan, Medlen.
Substitutes: Cringle, Fleming, Brett, Corness.
Attendance: 194.
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