The Iron's youthful development side were edged out in an eight-goal thriller at Cantley Park on Tuesday afternoon.
The hosts were in the ascendancy from the off and took the lead after a couple of acts of good defending from Scunthorpe. Sutton and Dean-Atkinson both defended well a minute prior to the opener before Joe Pugh netted after seven minutes.
Pugh planted a free header into the net from close range after a left-winged cross from Mandeville who broke the offside trap down the left.
Michael Carberry had a half chance to double the lead minutes later, but it was United who drew themselves into the ascendancy and might have equalised on sixteen minutes when Adelakun weaved his way past three men on the edge of the box before drawing Jones into a smart save. The ball, from there was loose, and Adelakun miss-kicked his follow-up which saw the ball run across the face of goal. Sutton retrieved it, but smashed an effort off the upright and back into play.
A minute later, Doncaster hit the woodwork at the other end of the pitch after Longbottom received the ball 25-yards from goal, with the ball hitting the top of the crossbar.
The Iron did equalise on twenty minutes though. United and Goode successfully defended a Rovers corner, the central defender clearing the ball which saw Dyche one-on-one with the last defender. The 18-year-old seized on the hesitancy to latch onto the ball and lift it over Jones and into the net.
United could have gone ahead through Adelakun six minutes later when Vose played him in on the right - only for his effort to skip wide of the post.
On 31 minutes, the hosts were awarded a penalty when the linesman deemed James Dean-Atkinson to have fouled in the area despite looking like he got a touch on the ball. The midfielder was booked and Jordan Linley dispatched the kick.
Matthew Davies was the player to go down under the challenge and he was replaced by Matthew Parkin.
Ledger was called into two saves soon after, denying Mandeville, but did well on both occasions to push the ball away from goal.
Just before the interval, Doncaster were denied a second penalty as Ledger looked to have brought down the attacker. Both the referee and linesman deemed there to be no contact and awarded a goal kick.
The host's lead was doubled seconds after the restart through Billy Whitehouse are his ferocious cross from the right flew into the roof of the net.
Jones was drawn into a save moments after the goal, conceding a corner, with Lewis Butroid volleying wide after the ball was cleared to him.
Lewis McMichael then did will on the right after an interchange from Vose and Adelakun. McMichael crossed from the right, but Dyche headed the ball over the bar.
The Iron made two switches with Goode and Adelakun making way for Leslie Sackey and Sonny Shilling respectively, with Dominic Vose halving the deficit shortly after.
He received the ball on the edge of the area, before curling an effort into the top right-hand corner from just over twenty yards out.
Three minutes later, that two-goal cushion was restored. Whitehouse did well on the right before advancing into the area and playing a square ball for Longbottom to prod home.
On 64 minutes, Linley was fortunate to remain on the pitch after he disagreed with a decision from the linesman. He was booked by the referee.
Four minutes late, substitute Shilling hit the post, slotting the ball past Jones after a flick on from Dyche and a square ball from Burdett.
Shilling did get his goal on 72 minutes though, elected to take a corner on the left. His cross curled goalwards and nestled in the top right-hand corner, with the 'keeper Jones left helpless.
A duo of substitutes from the hosts saw Joe Pugh and Connor Williamson replaced by Joshua Barker and Lloyd Henderson.
The final goal of the match saw the latter substitute turn home a flick on from Mbuti after a right-winged corner from the hosts.
Liam Mandeville then made way for Ryan Wanless for Doncaster with five minutes of the game remaining.
IRON: Ledger, McMichael, Goode (Sackey, 52), Butroid, Sithole, Sutton, Dean-Atkinson, Vose, Adelakun (Shilling, 55), Burdett, Dyche.
UNUSED SUBSTITUTE: Parker.