The Iron continued their impressive run away from home with a 1-1 draw at Coventry on Tuesday, meaning Scunthorpe are now unbeaten in nine matches on the road.
The result means that Mark Robins’ side drop down to 19th, six points clear of the bottom three and just eight points off the play-off places.
The Iron still have a couple of games in hand over some of the sides around them, with Coventry in 20th having played three more games than Scunthorpe.
Elsewhere in League One, Bristol City extended their lead at the top of the table to four points after their 3-1 win at home to Port Vale, coupled with their rivals losing.
Jay Emmanuel-Thomas, Joe Bryan and Matt Smith scored for the hosts, with Mark Marshall’s strike for Vale just a consolation.
Swindon fell to a 2-1 defeat away to Oldham, with two goals in as many minutes from Mike Jones and Conor Wilkinson proving decisive.
Massimo Luongo’s strike gave them a glimmer of hope, but defeat meant they fell slightly behind Bristol City although remain in second place. Oldham moved into fifth.
MK Dons had an early chance to move into the top two as they played Bradford on Monday evening, but lost 2-1 to the play-off chasing Bantams.
Highly-rated Dele Alli opened the scoring for the Dons, but Billy Clarke and James Hanson’s goals completed the turnaround for Bradford who are a point off the top six.
A Joe Garner brace helped Preston to move to within four points of the League One top two. Chesterfield had Sam Hird dismissed after just three minutes and have now dropped out of the play-offs.
Sheffield United capitalised by winning 4-1 at home to Colchester. Jose Baxter’s brace as well as goals from Matty Done and Jay McEveley sealed the win and left Colchester in the bottom four.
It was a disappointing evening for all the teams in the relegation zone, with all four sides falling to defeats.
Crawley lost 5-0 at home to Doncaster and also ended the evening with ten men. Andy Butler, Richie Wellens, Nathan Tyson, Harry Forrester and Curtis Main were all on target for Rovers.
Leyton Orient stayed bottom following a 1-0 defeat to Notts County, with Hungarian striker Balint Bajner’s goal the difference between the two sides.
Yeovil also fell to a 1-0 defeat, losing to Crewe who continue to climb away from the bottom four and now lie in 18th place.
There were 2-1 away wins for both Fleetwood, who won at Barnsley, as well as Gillingham who emerged victorious against Peterborough. Walsall also triumphed 3-2 in an entertaining clash with Rochdale.
Next up for the Iron is the visit of promotion chasers Swindon Town, with Mark Robins’ side looking to bounce back from defeat in their last home match to Oldham.